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Image Search Results
Journal: The Journal of Reproduction and Development
Article Title: MMP3 mediates E2-induced bovine endometrial cell proliferation by releasing HB-EGF
doi: 10.1262/jrd.2025-021
Figure Lengend Snippet: Expression of MMP3 in bovine endometrium and effects of E2, P4, and IFNα on MMP3 expression in BES and BEE. The expression of MMP3 in endometrial caruncular tissues (n = 5 per group) during the estrus cycle and early pregnancy was analyzed by RT-qPCR (A). Each mRNA expression normalized to GAPDH is shown as means ± SEM relative to follicle phase (= 1.0). Morphological features of BES (B) and BEE (C). The scale bar indicates 50 µm. Expression of MMP3 in BES and BEE was analyzed using RT-PCR (D). Effects of E2, P4, and IFNα on the expression of MMP3 in BES (E) and BEE (F) were also analyzed using RT-qPCR. The mRNA expression was normalized to GAPDH and shown as means ± SEM relative to the control (CT) (= 1.0). Different superscript letters indicate significant differences in each panel (P < 0.05). End, bovine endometrial tissue; NC, negative control without sample; CT, control without treatment; IFNα, human interferon α2.
Article Snippet: Cells were treated with 100nM 17β-Estradiol (E2; Sigma-Aldrich Co., Ltd., Tokyo, Japan), 1 μM Progesterone (P4; Sigma-Aldrich Co., Ltd.), and 50 IU/ml
Techniques: Expressing, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Control, Negative Control
Journal: The Journal of Reproduction and Development
Article Title: MMP3 mediates E2-induced bovine endometrial cell proliferation by releasing HB-EGF
doi: 10.1262/jrd.2025-021
Figure Lengend Snippet: Effects of E2, P4, and IFNα on the release of MMP3 in BES and BEE. Cells were cultured in the absence (CT) or presence of E2, P4, or IFNα for 48 h after confluence. The amount of MMP3 in the harvested supernatant was analyzed using casein zymography (A). Zymograms were quantified, and the activity of the precursor and mature forms was summed in BES (B). Each activity is shown as means ± SEM relative to the control (CT) (= 1.0). Different superscript letters indicate significant differences in each panel (P < 0.05). CT, control without treatment; IFNα, human interferon α2.
Article Snippet: Cells were treated with 100nM 17β-Estradiol (E2; Sigma-Aldrich Co., Ltd., Tokyo, Japan), 1 μM Progesterone (P4; Sigma-Aldrich Co., Ltd.), and 50 IU/ml
Techniques: Cell Culture, Zymography, Activity Assay, Control